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BactoCount
IBC 3.0

High-throughput multiplex flow cytometry for real-time bacteria & SCC in raw milk – scalable for future applications.

Milk bacteria counter accreditation
The BactoCount IBC 3.0 has been validated to be at least equivalent to the EN-ISO 4833-1:2013 and ISO 13366-1:2008 reference methods for the enumeration of microorganisms and somatic cells in 10 minutes for raw milk (cow, sheep, goat) – certificate no.: 2021LR97

Automated milk bacteria counter workflow for Total Bacteria (IBC) in raw milk

The BactoCount IBC 3.0 milk bacteria counter combines automated sample handling and flow cytometry in a robust 5-module design to deliver consistent Total Bacteria / Total Flora (IBC) results (raw milk total bacterial count) with ultra-low carry-over and full traceability.

  • Industrial control & traceability (PC + diagnostics)

    Continuous instrument monitoring with operator alerts, plus database storage of results and raw detector signals for retrospective review and recalculation after calibration.

  • Linear autosampler for routine high-volume testing

    Multiple rack formats, no compressed air, single-contact stirring, and intensive pipette washing (inside/out) for typical carry-over ~0.25% (ISO 16140 compliant).

  • Controlled incubation + dual ultrasonics for cleaner signals

    44-well incubator at 50°C with automated reagent dispensing; two ultrasonic probes reduce interferences (including somatic cells) to improve sensitivity and signal-to-noise.

  • Enclosed fluid handling for reagent safety and contamination control

    Real-time reagent level monitoring and 0.1 µm in-line filtration to protect reagent quality and minimize external contamination risks.

  • Temperature-regulated flow cytometer for stable measurement

    Compact closed unit with flow cell, optics, narrow band filter, one or two solid-state lasers, and sensitive photomultipliers for robust fluorescence pulse detection; gated pulses are converted to the official reporting unit via calibration/conversion equation.

Total flora proficiency testing – ISO 17043 (IBC)

Every month, we organize an international proficiency testing program on total bacteria (IBC), ISO 17043 certified and conducted in partnership with Actalia-Cecalait, the French reference laboratory.
This unique program ensures the optimal standardization of your BactoCount milk bacteria counter and guarantees the global equivalence of IBC and CFU results through our universal conversion equation.

The ultimate milk bacteria counter for high-throughput raw milk testing

The BactoCount IBC 3.0 is a fully automated, flow-cytometry milk bacteria counter built for rapid, highly accurate and reliable enumeration of individual bacteria and somatic cells in raw milk – ideal for mid- to large-size laboratories that require speed, standardization, and robust compliance. It delivers real-time Total Bacteria / Total Flora (IBC) with SCC in parallel, enabling fast payment testing, supplier monitoring, and early deviation detection, while preserving CFU/mL continuity via a conversion equation.

Multiplex & scalable platform designed for future applications

BactoCount IBC 3.0 was engineered as a multiplex, adaptable platform to accompany laboratories over the long term. Its configurable optical setup can include up to 2 lasers and up to 4 detectors (fluorescence + scatter), enabling multiplex signal acquisition and supporting multi-application capability as your lab’s needs expand. The software environment supports control, data acquisition, analysis, archiving and reporting, with a user interface that can be adapted depending on deployed applications—reinforcing the “future-proof” positioning for evolving programs and new testing requirements.

Technical Specifications*

Type of raw milk Cow, sheep, goat, …
Carryover ≤ 1% (typically ≤ 0.5%)
Total Flora ISO 16140-2:2016 compliant (According to ISO 16297; ISO 21187 and EURL MMP criteria)
Working range: 2 – 10,000 kIBC/mL
Measuring range: 5 – 100,000 kIBC/mL
Repeatability Range (kIBC/mL)
10 – 20
>20
Standard deviation of repeatability (Sr)
Sr ≤ 0,06 log
Sr ≤ 0,05 log
Accuracy Sy, x ≤ 0,3 log (versus ISO 4833)
Somatic cells ISO 16140-2:2016 compliant (According to ISO 8196-3; ISO 13366-2 and EURL MMP criteria)
0 – 10,000 thousand cells (kSCC)/mL
Accuracy ≤ 10%
Repeatability Range (kSCC/mL)
50 – 300
300 – 500
> 500
Relative std. dev. repeatability (Cv)
≤ 5%
≤ 3%
< 2%
Additional technical specifications
Work factor (undiluted) 100
Speed 100 samples/hour
200 samples/hour
Voltage 115/220 V | 50/60 Hz
Dimensions (WxHxD) 152.4 x 121.9 x 61.0 cm
Weight 115.0 kg
Connected to the local database and accessible remotely

*Specifications subject to change without notice.

MAINTENANCE & SUPPORT

Customer service has always been a priority for Bentley Instruments. We guarantee our customers prompt assistance for optimum productivity.

In order to guarantee the highest level of productivity, our team of technicians takes care of installation, training and diagnostics remotely and locally.

The FreshDesk platform is available by registering and gives you access to our e-service platform: e-ticket service to ask our engineers about your problems, technical databases (equipment history, technical notes, manuals...), diagnostic tools (problem/solution), worldwide network of production engineers and after-sales service for a quick and interactive assistance at your disposal at any time.

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    BENTLEY INSTRUMENTS EU

    ZA Brunehaut 840 rue Curie
    62161 Maroeuil - France
    +33 (0)2 85 52 90 73
    Fax : +33 (0)3 20 09 87 12
    info@bentleyinstruments.eu

    BENTLEY INSTRUMENTS INC.

    4004 Peavey Rd Chaska
    Minnesota 55318 - USA
    +1 952 448 7600
    +1 952 368 3355
    info@bentleyinstruments.com